Les antibiotiques sont produits sous des formes pharmaceutiques telles que des pilules antibiotiques en ligne
elles permettent d'injecter la quantité de préparation strictement nécessaire.
Efficient usage of hydrogen peroxide in tetracycline degradation via photo-fenton process
Enhanced photo-Fenton Process for Tetracycline Degradation using
Efficient Hydrogen Peroxide Dosage
Evelyn Yamal-Turbay*, Esther Jaén, Moisès Graells, Montserrat Pérez-Moya
Departament d'Enginyeria Química, EUETIB, Universitat Politècnica de Catalunya, c/Comte
d'Urgell, 187, 08036 Barcelona, Spain
The remediation of water solutions containing Tetracycline antibiotic (TC) using photo-Fenton
treatments has received scarce attention in the literature. However TC deserves attention due to its
condition of emerging contaminant. In this work, TC oxidation in water solutions (12 L, 40 mg L-1) by
means of photo-Fenton reaction under variable hydrogen peroxide dosage is investigated. This shows
a more efficient use of the hydroxyl radicals produced in the reaction medium and enhances treatment
performance. First, a suitable range of Fenton reagent loads is determined in a preliminary study. The
hydrogen peroxide dosage is parameterized through two variables: the initial release of the total load,
(kick-off), and the time tini
at which the continuous dosage of the rest of the load starts. Hence, a
design of experiments (22) can be used to characterize the performance of the process under different
hydrogen peroxide dosage protocols. The results obtained this way show that total TC remediation is
attained in all the cases studied, but alike amounts of hydrogen peroxide lead to total mineralization
only when the dosage scheme is conveniently tuned. Therefore, the photo-Fenton treatment has proved
to be effective in removing TC from water solutions, and the opportunity for an efficient dosage to
reduce the requirements of hydrogen peroxide has also been confirmed.
Tetracycline antibiotic, emerging contaminants, photo-Fenton treatment, hydrogen
peroxide dosage, design of experiments.
* Corresponding author: Escola Universitària d'Enginyeria Tècnica Industrial de Barcelona. Comte d'Urgell 187. 08036 Barcelona, Spain. +34 934137275. email@example.com
Tetracycline in water solutions is shown to be totally degraded via photo-Fenton treatment
Subject to dosage, a H2O2 load can mineralize a tetracycline solution to different extents
H2O2 dosage is modeled via two parameters, upon which a design of experiments is proposed
Design of experiments dosage conditions yield mineralization rates ranging from 77% to almost
If conveniently dosed, stoichiometric H2O2 loads can achieve total mineralization
In recent years, a new group of contaminants called emerging contaminants has been detected in
water sources. Although they are found in very low concentrations (in the order of ng-μg L-1) they can
become a real environmental problem because of their high persistence and ecotoxicity [1, 2]. Among
these emerging contaminants are pharmaceuticals and personal care products (PPCPs) that can act as
endocrine disruptors and affect bacteria used in conventional water treatments .
In particular, tetracyclines (TCs) are broad-spectrum antibiotics with activity against gram-positive
and gram-negative bacteria. The presence of such antibiotics in the environment is mainly due to their
discharge through excretion whether or not metabolized. As conventional treatments cannot be
expected to process this kind of contaminants, they come to appear in surface water, groundwater and
even in drinking water, developing antibiotic-resistant pathogens, among several other environmental
issues [3-5]. According to Wang et al.  they have been detected at concentrations at 0.07-1.34 µg
L-1 in surface water samples.
In the last thirty years, advanced oxidation processes (AOPs) have acquired increasing interest as
an option either to remediate recalcitrant contaminants before or after conventional treatments [7-9].
AOPs consist in the in situ generation of highly oxidant species to degrade organic matter, and have
shown to provide excellent results, especially those processes based on the use of the Fenton reagent.
Fenton and related processes (photo-enhanced Fenton process, electro-Fenton, sono-Fenton and so on)
have proved to be efficient in mineralizing antibiotics [3, 5, 10, 11].
Tetracycline degradation by means of AOPs has been investigated in the past, especially by using
UV and UV/H2O2 treatments, and heterogeneous photocatalysis, (contaminant concentrations ranging
from 10-100 mg L-1). UV has shown to be effective in degrading this contaminant and the presence of
H2O2 has revealed to increase TC degradation and mineralization rates [9, 12-45]. Heterogeneous
photocatalysis with TiO2 has also been investigated, and a lower performance with respect to UV and
UV/H2O2 processes has been reported [8, 16]. Electrochemical processes have also shown to be
effective for TC degradation [17, 18]. Conversely, TC degradation via photo-Fenton process has
received scarce attention, although TC is among the components of some wastewaters whose photo-
Fenton treatment has been investigated  and the research by Rossi and Nogueira  reported total
remediation of 24 mg L-1 TC solutions.
In Fenton processes, hydroxyl radicals are produced by the reaction between hydrogen peroxide
and a ferrous salt . In the specific case of photo-Fenton reaction, an external source of energy (UV
irradiation) can enhance the process by accelerating iron ions to be regenerated (from Fe3+ to Fe2+) and
recycled . However, since the hydroxyl radical is extremely unstable and non-selective, it is
frequently scavenged by undesired secondary reactions. This indicates the convenience to
continuously produce hydroxyl radicals in the reaction media instead of using high hydrogen peroxide
concentrations at the beginning of the process .
Recent efforts have been devoted to the use of continuous or semi-continuous hydrogen peroxide
dosage for reducing the scavenging of hydroxyl radicals, thus reducing costs and environmental
impact as well [21, 23]. Examples of this are the work by Chu et al. , who proposed a stepwise
dosage of hydrogen peroxide that improved mineralization; the work by Zazo et al. , who applied
a continuous dosage protocol to the Fenton treatment to increase abatement of TOC with the same
overall H2O2 consumption; and the work by Prato-Garcia and Buitrón , who addressed the
decolorization of azo dye mixtures by means of two different continuous dosage strategies that
improved effluent quality in terms of toxicity and biodegradability. Hydrogen peroxide dosage has
been investigated for Fenton treatments aimed at increasing the efficiency of the remediation of
solutions of other six selected antibiotics . However, regarding the oxidation of TC antibiotic in
water solutions via photo-Fenton treatment, to the best of the authors' knowledge, no study has been
reported on the influence of hydrogen peroxide dosage.
More recently, Yamal-Turbay et al.  proposed a practical parameterization of hydrogen
peroxide dosage protocol that allowed setting an easy design of experiments to characterize the
influence of hydrogen peroxide dosage on the performance of a photo-Fenton treatment. This
technique was tested in the remediation of coffee solutions and led to the determination of more
efficient dosage conditions for hydrogen peroxide and enhanced process performance. This work aims
to apply this systematic dosage protocol for improving the performance of the photo-Fenton treatment
of TC solutions in water.
95% purity TC hycrochloride from Sigma Aldrich was purchased to prepare 40 mg L-1 solutions in
tap water. Fenton reagents: hydrogen peroxide 33% w/v (Panreac) and 7-hydrated iron (II) sulfate
(Merck) were used as received. HCl (J.T. Baker) and NaOH (Panreac) 1 M were used for automated
Oxalic acid 0.01 M (Panreac) prepared in filtered milli Q grade water and J.T. Baker ultragradient
HPLC acetonitrile and methanol were used as HPLC mobil phases, while a stock of ammonium
metavanadate (Riedel-de-Haën) 0.062 M in H2SO4 (Panreac) 0.58 M according to Nogueira et al. 
was kept refrigerated for hydrogen peroxide measurement.
2.2 Pilot plant description
The photochemical pilot plant consists in a 12 L system: glass reservoir and piping system. The
light source is a 230 W medium pressure mercury lamp UV TQ718 (irradiating from 300 to 550 nm)
placed in a glass cooling jacket in the center of the reservoir. The incident photon flux, determined by
potassium ferrioxalate actinometry , was 3.4033× 10−5 Einstein s−1.
The system is provided with a centrifugal pump which keeps a recirculation flow of 12 L min-1.
The reaction pH was maintained at 2.7±0.1 by using a proportional-integral-derivative (PID)
controller. Figure 1a shows a diagram of the pilot plant.
2.3 Analytical methods
Process performance was evaluated by withdrawing aliquots from the pilot plant and measuring the
following variables at regular time intervals:
Total organic carbon (TOC) concentration was measured by means of a Shimadzu VCHS/CSN
TC concentration was determined via HPLC, using an Agilent 1200 series with UV-DAD
array detector. The chromatographic conditions and data analysis were performed using the
Agilent Chem-Station (Rev.B.04.06 SP1) software. The chromatographic column was a
5 µm 4.6x150 mm Zorbax Eclipse XDB-C18 (Agilent Technologies), maintained at 30ºC, and
the diode array detector was set at 271 nm. 20 μL samples, injected by a manual injector, were
eluted by a mixture (70% 0.01 M oxalic acid, 10% methanol and 20% acetonitrile) flowing at
1.0 mL min-1 . Tetracycline retention time under these conditions was 2.4 minutes. A five-
level calibration curve (range 2-30 mg L-1) was used for TC quantification.
H2O2 concentration was measured by a UV-vis spectrophotometer Hitachi U-2001 after
reaction with ammonium metavanadate, following the technique proposed by Nogueira et al.
2.4 Hydrogen peroxide dosage definition
The continuous dosage of H2O2 was executed by means of a peristaltic pump (Watson Marlow 300
series OEM) programmed from the pilot plant SCADA. The dosage protocol was previously proposed
and stated  and is characterized (Eq. 1) by the fraction of the total dose of H2O2 added initially, y0
the dosage interval, tadd
, and the time at which the dosage starts, tini
. Figure 1b shows a graphical
representation of the addition protocol.
After a preliminary study which will be explained in further sections, the total amount of H2O2 was
fixed to 858 mg, which corresponds to an equivalent concentration of 71.5 mg L-1. The treatment span
and the dosage interval were also fixed (TS
=90 min; tadd
=30 min). Hence, the remaining two degrees
of freedom (y0
) were used in the design of experiments. The quantitative performance index
selected to rank the output of the assays and to discuss the results is the conversion attained at TS
defined as follows:
3. Results and discussion
3.1. Preliminary assays
3.1.1. Reagent doses
As mentioned in the introduction, the concentrations investigated in previous works concerning TC
degradation by means of photolysis and photocatalysis processes ranged between 10 and 300 mg L-1
[4, 8, 13, 16], while Rossi and Nogueira  proved that photo-Fenton process can be successfully
applied for the degradation of 24 mg L-1 TC solutions with hydrogen peroxide and ferrous iron initial
concentrations ranging from 35 to 680 mg L-1 and 5.6 and 11 mg L-1, respectively. According to this
information and taking into account TOC analyzer sensibility, TC concentration was fixed at 40 mg
L-1, which corresponds to a TOC concentration of 23 mg L-1 and requires a stoichiometric H2O2
concentration of 150 mg L-1.
A 22 factorial design of experiments (DOE) with star points was performed to decide Fenton
reagent doses. It was important to identify a dose which permits a compromise between degradation
and dosage interest. For that aim, Fe2+ dose was centered at 5 mg L-1 (10 mg L-1 is the maximum legal
value in effluents in Spanish legislation ), having minimum and maximum values 2 and 8 mg L-1,
Regarding H2O2 load, this ranged between 9% and 90% of the stoichiometric amount given by the
TC oxidation just by means of hydrogen peroxide, which is taken as a reference. Sub-stoichiometric
hydrogen peroxide loads can be used to ease the identification intermediate species at the early stages
of the reaction ; furthermore, sub-stoichiometric loads can be enough for total TC oxidation since
hydrogen peroxide is not the only source of oxygen [21, 34]. Minimum and maximum values of the
H2O2 load were set at 14.3 and 128.7 mg L-1, having a center value of 71.5 mg L-1, which corresponds
to a 48% of the stoichiometric amount. Figure 2a presents DOE results considering TS
as the system
Low iron loads (2 mg L-1) provide degradation rates around 55% despite H2O2 dose (red line). On
the contrary, higher iron values (5 or 8 mg L-1) noticeably increase response values as H2O2 doses
increase. However, 5 mg L-1 iron loads lead to higher TOC degradation than 8 mg L-1 for equal H2O2
amounts (lines blue and green). Thus, 5 mg L-1 iron loads offer more efficient use of the hydroxyl
radicals generated in the system. According to this, iron dose was set at 5 mg L-1.
Finally, a hydrogen peroxide dose was set at 71.5 mg L-1 because higher values, when combined
with 5 mg L-1 iron doses provide too fast TOC reduction, which complicates the evaluation of the
effect of reagent dosage on degradation. In brief, from here on, the following concentrations are used:
Tetracycline initial concentration = 40 mg L-1
Hydrogen peroxide equivalent concentration = 71.5 mg L-1
Iron (II) initial concentration = 5 mg L-1
3.1.2. Blank assays
A set of blank assays was initially performed in order to evaluate the influence of each separate
reagent on TOC and TC degradation, which are next presented. Figure 2b shows the evolution of
TOC, H2O2 and TC concentrations for UV treatment, UV/H2O2, Fenton and photo-Fenton, involving
the previously stated reagent doses when applied.
Mineralization by means of UV treatment only reaches 35%, UV/H2O2 and Fenton allow almost
60%, and the photo-Fenton process may reach 77%. TC is completely degraded in all the cases where
H2O2 is present, being the degradation faster when the ferrous salt is added. On the contrary, only a
40% of TC is degraded by solely using UV irradiation (90 min treatment). Both TC and TOC
reduction rates obtained without using H2O2 are comparatively poor. However, it is important to bear
in mind that these rates indicate that, to a non-negligible extent, H2O2 may not be the sole responsible
of the complete oxidation of organic matter.
Once established that the photo-Fenton process is capable of degrading TC and mineralize organic
matter, H2O2 dosage was investigated in order to determine to which extent process efficiency can be
improved given the same reagent loads.
3.2. DOE for dosage characterization
A design of experiments considering the dosage parameters y0
(eq.1) and the response TS
was applied; the earliest sample time at which TC is not detected (tTC
) was also included as a response.
Table 1 lists the levels and results of the planned assays according to a 22 DOE with start points and
three center points for statistical validity; minimum and maximum factors were set at 10 and 30% for
and 0 and 30 minutes for tini
. Reference experiments were included in the study.
Figure 3a shows the results of the central experiment of the design, which was repeated six times.
Average values are presented along with the corresponding standard deviations. Total remediation of
the sample is shown to be achieved with these conditions. Regarding TC degradation, it was achieved
within 10 minutes treatment.
From this point on, experiments are named according to the following nomenclature: "Experiment-
". Assay R corresponds to the reference experiment (no dosage protocol).
Figure 3b compares the evolution of TOC and TC concentrations for the central assays (y0
=15 min), an assay without kick-off (experiment code L, y0
=0 min), and the reference
assay. The performance obtained is analogous to that obtained by previous studies using caffeine .
Only 77% mineralization is achieved without dosage. However, the proposed scheme, when
conveniently tuned, shows that it is possible to reach up to almost total mineralization (98%), which
corresponds to 27 percent points of improvement. According to Prato-Garcia and Buitrón , this
may be explained because when the entire reagent dose is added at once, the excess of H2O2 promotes
secondary reactions that scavenge hydroxyl radicals and "waste" them. Dosage allows reducing this
excess and having a more efficient dedication of hydroxyl radicals to degrading organic matter [22,
Regarding TC remediation, table 1 shows the earliest sample time (tTC
) at which TC is not detected
and total degradation is assumed. The slowest degradation is observed in experiments B and J, for
increases up to 45 and 30 minutes respectively, while for the rest of the assays tTC
15 minutes. This behavior can be explained because of the combination of low initial hydrogen
peroxide doses (y0
), which is rapidly exhausted and causes the process to stop, and the late dosage
), which slows the process down; actually, better performance of experiment J compared to assay B
obeys to sooner tini
despite lower y0
Figure 4 shows the relation between conversion at TS
, and the time at which total degradation
is assumed, tTC
. This confirms the opportunity to improve the treatment outcome by adjusting the
dosage parameters. When the reagent is dosed proportionally without kick-off addition (experiment
L), an improvement of 18 percent points is possible; furthermore, both objectives, maximizing
mineralization and minimizing time for degrading TC, are achieved simultaneously. That is the case of
experiment C, which is the closest to the low right corner of the plot.
Regarding the evident interaction between both factors y0
these results also indicate the
higher significance of the latter. When continuous dosage starts soon, better performance is achieved
; conversely, low y0
values may require starting dosage at earlier tini
values. Further data on
the influence of low y0 values on TC degradation are given in Figure 5. Compared to assay J (tini
min), the higher y0
value of new experiment P_8_15 (8%) produces no performance changes;
however, the lower y0
value of new experiment Q_4_15 (4%) results in hydrogen peroxide exhaustion
and the process interruption until more reagent is dosed after 15 minutes.
The oxidation of TC antibiotic in water solutions by means of the photo-Fenton treatment was
investigated. The treatment was applied to 12 L of 40 mg L-1 tetracycline samples with total reagent
doses of 71.5 mg L-1 hydrogen peroxide (48% of stoichiometric dose) and 5 mg L-1 Fe2+ (half the legal
limit in wastewaters in Spain). These conditions achieved total TC remediation and to produce up to
77% solution mineralization within the reaction span studied.
Hydrogen peroxide dosage was also investigated in order to improve process performance. Since
excess hydrogen peroxide fosters scavenging reactions, dosage is considered to provide the conditions
for reducing this unsolicited effect. A dosage protocol introduced in a previous work was applied to
the study of the degradation of TC.
The systematic parameterization of the dosage led to a DOE for determining the treatment outcome
for a given set of assays. Hence, given this fixed H2O2 load, treatment performance was shown to be
able to improve total mineralization (up to 22.6 percent points) when dosage parameters were
The efficient use of hydrogen peroxide, via dosage, has revealed to significantly improve the
performance of the treatment of water solutions containing TC by cutting the H2O2 demand and
reducing the cost of photo-Fenton treatments. This work has also shown that the H2O2 demand can be
reduced quite below the stoichiometric value, which suggests the importance of other oxygen sources
in the photo-Fenton treatment. This additional aspect and its relation with dosage deserve further
The authors would like to thank the Spanish Ministerio de Economía y Competitividad and the
European Union (through the European Regional Development Fund) for supporting this research,
which is part of the activities developed under the Project EHMAN (DPI2009-09386) and Project
SIGERA (DPI2012-37154-C02-01). Evelyn Yamal-Turbay also thanks Universidad de Carabobo for
financial support through professorial grant CD-4352.
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PD Dr med WP Bieger NeuroStress Guide EINLEITUNG Der vorliegende NeuroScience-Guide ist als Anleitung für Patienten, Ärzte und Therapeuten gedacht. Er soll einen Einblick in die Funktionsweise des Neuroendokriniums und in die Grundlagen neuroendokriner Funktionsstörungen und deren Behandlung vermitteln. Die von uns entwickelte „NeuroStress"-Diagnostik wird vorgestellt und physiologische Behandlungsformen besprochen. Schon lange gibt es hochwirksame Substanzen für die natürliche Behandlung psychoneurovegetativer Störungen, die allerdings durch die Entwicklung der modernen Psychopharmaka verdrängt wurden. Die unbefriedigenden Ergebnisse der Antidepressiva haben die traditionellen Behandlungskonzepte jedoch wieder ins Bewusstsein gerückt. Unser aktuelles ganzheitliches diagnostisch-therapeutisches Konzept greift die bewährten Verfahren auf und verbindet sie mit innovativen Diagnose- und Behandlungsformen aus den USA. Eingangsüberlegungen: 1. Die Zahl neurovegetativer Störungen und psychischer Krankheiten nimmt weltweit stark zu. Damit auch die Nachfrage nach neuen diagnostischen Möglichkeiten und effzienten, gut verträglichen Behandlungen. Seit Jahren steigt die Zahl psychischer Störungen in den westlichen Industrieländern. Man geht davon aus, dass bis zu 60% der Krankheitsfälle in der täglichen ärztlichen Praxis psychischer Natur sind bzw. eng mit psychischen Belastungen verbunden sind. Schon heute entfallen viele Krankheitstage auf psychische Störungen, ihre Zahl nimmt ständig zu, während die Gesamtzahl krankheitsbedingter Fehltage seit Jahren zurückgeht. Besonders gravierend ist die Zunahme der Depressionen. Während Herz-Kreislauferkrankungen, Herzinfarkt, sogar die häufigsten Krebserkrankungen (Lungen-, Brust- und Prostatakrebs) seit einigen Jahren abnehmende Tendenz zeigen, nimmt der Anteil von Depressionen ständig zu. Die WHO geht in einem ihrer jüngsten Gesundheitsberichte (2006) davon aus, dass bereits in den nächsten 5-10 Jahren Depressionen die zweithäufigste medizinische Krankheitsursache überhaupt sein werden. 2. Psychopharmaka werden heute in enormem Maße eingesetzt, sie sind mit >65 Mrd € die umsatzstärkste pharmazeutische Präparategruppe. Ihre Wirksamkeit, vor allem die der Antidepressiva, ist jedoch begrenzt. Die Nebenwirkungen sind zahlreich und zum Teil lebensbedrohlich. Immer wieder werden Zweifel am Aussagewert von Psychopharmakastudien geäußert, die Publikation von klinischen Studien mit Antidepressiva erfolgt offensichtlich nach willkürlichen Kriterien (NEJM, 2008). In einer kürzlichen Metaanalyse wurde die fehende Wirksamkeit von Antidepressiva bei leichten bis mittelschweren Depressionen konstatiert (PloS Medicine, 2008). Nur bei schweren Depressionen findet sich ein Vorteil von Antidepressiva gegenüber Placebos. 3. Die Neurobiologie psychischer Störungen wird seit Jahren intensiv untersucht, neue Untersuchungsverfahren wie MRT, PET, SPECT, Immunologie oder Molekulargenetik haben das Verständnis der zentralen Prozesse enorm verbessert. Neue Diagnose- und Behandlungsverfahren können daher stärker auf Wissen und weniger auf Zufallsbeobachtungen wie bisher basiert werden. STRESS Als eines der zentralen Geschehen gilt chronischer Stress, der vielfach zu bleibenden psychovegetativen Störungen führt. Ein kürzlich erschienenes Buch des Bonner Psychiaters Prof. Benkert (2006) hat den Begriff der „StressDepression" geprägt und damit „Stress" als ganz wesentlichen Grund für psychische Störungen einschließlich Depressionen in den Mittelpunkt
By Xiaoni Zhang and Victor R. Prybutok MARKETS DIFFER IN CHINA, THE U.S., AND EUROPE Market differences include communications standards, price structures, government regulations, customer demographics, usage patterns, business potential, and technology adoption strategies. Despite the 2001 downturn in the global telecommunica-tions market, the mobile penetration rate in China is increas-ing due to the elimination or reduction of mobileconnection fees. The mobile market worldwide is dynamicin terms of technology development, and competition isaggressive. The potential size and growth rate of the Chinese,U.S., and European mobile markets warrant examination ofthe state of their wireless development. Here, we explore thatgrowth by examining the differences among the threeregions, especially those in mobile consumer demographics,Short Message Service (SMS) usage, and 3G technology.