|

Veritastk.co.jp

MiRA Test
Microbiological test with spores of Geobacillus stearothermophilus for the detection of antibiotic and sulphamide residues in milk DESCRIPTION
Antimicrobial substances are given to cattle for therapeutic treatment of infections and so they can be present in milk as residual
drugs. The presence of residual antimicrobial drugs in milk is a potential hazard for the consumers since those substances interfere
with the intestinal flora, can cause allergic reactions and can contribute to the spreading of resistant microorganisms.
Furthermore, the presence of antimicrobial drugs in milk is a serious technological problem for dairy industry, since antimicrobial
substances interfere with milk fermentation which is the basis of many dairy productions.
The detection of inhibiting substances in milk is ruled in Europe by EEC 91/180 and by Regulation EEC 2377/90 and following
modifications.
MiRA (Milk Rapid Antibiotic) Test is a microbiological test, set for the detection of residual antibiotics and sulphamides in milk, that
uses spores of Geobacillus stearothermophilus and a culture medium designed for that purpose.
CONTENT OF THE PACKAGE
Each package contains:
• n. 50 tubes containing liquid culture medium
• n. 50 discs containing spores of Geobacillus stearothermophilus
• 1 instructions sheet
PRINCIPLE OF THE METHOD
The test detects residues of antibiotics and sulphamides through their inhibition action against the growth in vitro, at 64 ± 0,5 °C, of
Geobacillus stearothermophilus. The inhibition of bacterial growth is made evident by the presence of a fermentable sugar and a pH
indicator in the culture medium.
PROCEDURE FOR USE
• Remove the cup from the tube and add 1 disc of spores. Shake gently.
• Put the cup and introduce the tube in a waterbath at 64 ± 0,5 °C or in a Termoblock set at 64 ± 0,5 °C. Incubate for 20'.
• Remove the tube from the incubator and let it reach room temperature (roughly 5').
• Remove the cup and introduce 1 mL of the milk under test.
• Prepare one or more analyte-positive controls, by the addition of antibiotic/sulphamide into the tubes as indicated in the paragraph
"Quality Control for the user".
• Put the cup to the tube and let the antibiotic/sulphamide, if present, act at room temperature for 20'.
• Reintroduce the tube in the waterbath or in the Termoblock at 64 ± 0,5 °C for the second incubation for 3h - 3h 30'.
INTERPRETATION OF THE RESULTS
At the end of the second incubation observe colour of the liquid in the tubes that, if green, indicates antibiotic/sulphamide residues in
the examined milk in concentrations above the detection limits; otherwise, a yellow colour indicates the absence of
antibiotic/sulphamide residues in the examined milk or their presence in concentrations under the detection limits.
Photo 1. Results obtained by MiRA Test after 3h 30' of incubation at 64 ± 0,5 °C:
• in the left side, no colour change of the pH indicator: analyte-positive milk sample containing antibiotic/sulphamide residues in
concentration above the detection limits; • in the right side, colour change of the pH indicator: analyte-negative milk sample containing no antibiotic/sulphamide residues or containing antibiotic/sulphamide residues in concentration under the detection limits.
USER QUALITY CONTROL
Every batch of MiRA Test is submitted to quality control. The analyte-negative control is obtained using a certified milk sample free of
antibiotics. The analyte-positive controls are obtained using milk samples with the addition of antibiotics/sulphamides such as
gentamicin (100 µg/kg), penicillin G (4 µg/kg) and sulphadiazine (150 µg/kg).
PRECAUTIONS
The product MiRA Test is not classified as dangerous under current legislation. MiRA Test is a disposable device to be utilized only
for in vitro use, it is intended for use in a professional environment and must be used in laboratory by properly trained personnel, using
approved asepsis and safety methods for handling pathogenic agents.
STORAGE
Store MiRA Test at 2-8 °C in its original packaging. Keep away from heat sources and avoid excessive temperature changes.
In such conditions MiRA Test will remain valid until the expiry date indicated on the label. Do not use beyond that date.
Eliminate without using it if there are deterioration signs.
DISPOSAL
After use and before disposal, tubes must be properly decontaminated with chemical substances or autoclaved.
BIBLIOGRAPHY
• DM 26/03/1992 GU n° 90 16/04/1992: Attuazione della decisione n. 91/180/CEE concernente la fissazione di metodi di analisi e prova relativi al
latte crudo e al latte trattato termicamente.
• CONALAnalisi e controllo qualità dei prodotti lattiero-caseari Il latte VIII/5, 356-357 (1983).
• 91/180/EEC: Commission Decision of 14 February 1991 laying down certain methods of analysis and testing of raw milk and heat-treated milk. Official Journal L 093 , 13/04/1991 P. 0001 - 0048.
• Van Oss J.L., Lameris S.A. Doodewaard J., Oostendrop J.G., Diffusion Test for the determination of antibiotic residues in milk. Neth. Milk Dairy J. 29, 16 (1975).
• International Dairy Federation International Standard FIL-IDF 57:1970 Detection of penicillin in milk by a disk assay technique (1970).
• Deborah Currie, Lorraine Lynas, D. Glenn Kennedy and W. John McCaughey. Evaluation of a modified EC Four Plate.
• Method to detect antimicrobial drugs. Food Additives and Contaminants, 1998, Vol. 15, No. 6, 651-660.
• Regulation 2377/90 ff EEC.
MiRA Test
Table 1: Sensitivity of MiRA Test to some antibiotics/sulphamides, commonly used in veterinary medicine and respective MRL
(Maximum Residue Limits) values valid for Europe1.
Table 1
MRL for milk 1
MiRA Test Sensitivity in 3h 30'
MAXIMUM RESIDUE LIMITS (µg/kg) DETECTION LIMITS (µg/kg) AMINOGLYCOSIDES
Gentamicin
BENZIL PIRIMIDINE
Trimethoprim
1 Regulation 2377/90 ff EEC.
TABLE OF SYMBOLS
Contains sufficient for Caution, consult Catalogue number Rev.2 / 01.11.2007 LIOFILCHEM
Via Scozia Zona Ind.le - 64026 Roseto D.A. (TE) - Italy
Phone +390858930745 Fax +390858930330 Website:E-Mail: [email protected]

Source: http://www.veritastk.co.jp/attached/876/80355.pdf